Coding
PduD40-Lim

Part:BBa_K562015:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-18)


Salty_PduD40-Limonene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 239
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1937
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 239
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 341


Design Notes

A HindIII site from the limonene synthase gene was removed by site-directed mutagenesis. This will allow future cloning into other compatible vectors.


Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and biobrick BBa_I742111.

References